Hi L Keller,   No, your meals on Monday would not affect your lab results on Wednesday.  A fasting test (no foods/drinks 8-12 hours prior to labs) gives you the most accurate results.  If your labs had been non-fasting then there is the possibility for false highs.   A total cholesterol of 226 is not cause to panic, but a good wake up call to make some changes to lower your levels.  Click here to learn more about a diet to lower cholesterol and keep up the physical activity.  Also, discuss your results with your MD.   All the best,   Lisa Nelson, RD, LN The Heart of Health - Heart Health and Weight Loss Tips